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    1. SARS-CoV-2 Spike RBD Nanobody

      Datasheet
      • 货号:
        CSB-RA33245A2GMY
      • 规格:
        ¥4,800
      • 图片:
        • The Binding Activity of SARS-CoV-2 Spike RBD Nanobody with SARS-CoV-2-S1-RBD
          Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD () at 2 μg/ml can bind SARS-CoV-2 Spike RBD Nanobody, the EC50 is 0.8674 ng/ml.
        • In the Colloidal Gold Immunochromatography Assay
          detection system, the background of antibody (CSB-RA33245A2GMY) is clean, the detection limit can be as low as 25ng/ml (1.75ng/0.07ml), and the sensitivity is very good.
        • Binding signal of SARS-CoV-2-S1-RBD () and ACE2 protein-HRP conjugate () was inhibited by SARS-CoV-2 Spike RBD Nanobody (CSB-RA33245A2GMY) with the ICC50 is 1.296 nM.
        • Binding signal of SARS-CoV-2-S1-RBD () and ACE2 protein-HRP conjugate () was inhibited by SARS-CoV-2 Spike RBD Nanobody (CSB-RA33245A2GMY) with the IC50 is 0.1074 μg/ml.
        • SARS-CoV-2 Spike protein RBD His/Sumostar Tag () captured on COOH chip can bind SARS-CoV-2 Spike RBD Nanobody (CSB-RA33245A2GMY) with an affinity constant of 28.2nM as detected by LSPR Assay.
        • ELISA: Immobilize various types of SARS proteins at concentration of 2μg/ml on solid substrate, then react with SARS-CoV-2 Spike RBD Nanobody at concentration of 100μg/ml, 10μg/ml and 1μg/ml. It shows the SARS-CoV-2 Spike RBD Nanobody (CSB-RA33245A2GMY) is specific for SARS-CoV-2-S1-RBD protein, without any cross-reactivity with MERS-CoV, SARS-CoV, HCoV-OC43 or HCoV-229E.
      • 其他:

      产品详情

      • 产品描述:

        This SARS-CoV-2 S1-RBD (Spike Glycoprotein S1 receptor-binding domain) antibody is a recombinant monoclonal antibody (also a Nanobody) generated through the expression of a DNA sequence inserting a human IgG1 Fc domain at the C-terminus, in human embryonic kidney 293 cells (HEK293). The DNA sequence encodes the SARS-CoV-2 spike RBD. The antibody is purified by protein G in vitro. It has been validated with high reactivity towards SARS-CoV-2 S1-RBD by a functional ELISA and good sensitivity for human SARS-CoV-2 spike glycoprotein (S protein) via the Colloidal Gold Immunochromatography Assay (GICA).
        It is also validated in Neutralizing and LSPR. In neu assay, the binding signal of the SARS-CoV-2 S1 RBD antibody was inhibited by ACE2 protein-HRP conjugated inhibitor, with a 0.1074 μg/ml IC50. In LSPR assay, the SARS-CoV-2 S1 RBD antibody showed a high affinity with SARS-CoV-2 Spike protein RBD (affinity constant: 28.2nM).
        Specifically binding and recognizing the RBD of the SARS-CoV-2 spike glycoprotein, the SARS-CoV-2 S1 RBD antibody can react with samples infected with human coronavirus SARS-CoV-2. But it does not respond to MERS or SARS-CoV spike protein. Akin to other nanobodies, this recombinant nanobody is small and stable, which allows for its reaching to hidden epitopes such as crevices of target proteins.

      • Uniprot No.:
      • 别名:
        Anti-coronavirus spike Antibody; Anti-cov spike Antibody; Anti-ncov RBD Antibody; Anti-ncov S1 Antibody;Anti-ncov spike Antibody; Anti-novel coronavirus RBD Antibody; Anti-novel coronavirus S1 Antibody; Anti-novel coronavirus spike Antibody; Anti-RBD Antibody; Anti-S1 Antibody; Anti-Spike RBD Antibody; E2 Antibody; E2 glycoprotein Antibody; Human coronavirus spike glycoprotein Antibody; S Antibody; SARS-CoV-2 S1 RBD Antibody; S glycoprotein Antibody; Spike glycoprotein Antibody
      • 反应种属:
        Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
      • 免疫原:
        Recombinant Human Novel Coronavirus Spike glycoprotein(S) (319-541aa) (CSB-YP3324GMY1 and CSB-MP3324GMY1b1)
      • 免疫原种属:
        Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
      • 标记方式:
        Non-conjugated
      • 克隆类型:
        Monoclonal
      • 抗体亚型:
        VHH fusion with human IgG1 Fc
      • 纯化方式:
        Affinity-chromatography
      • 克隆号:
        A1
      • 浓度:
        It differs from different batches. Please contact us to confirm it.
      • 保存缓冲液:
        Preservative: 0.03% Proclin 300
        Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
      • 产品提供形式:
        Liquid
      • 应用范围:
        ELISA, GICA, Neutralising
      • 推荐稀释比:
        Application Recommended Dilution
        ELISA 1:10000-1:100000
        GICA 1:10000-1:40000
        Neutralising 1:100-1:10000
      • Protocols:
      • 储存条件:
        Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
      • 货期:
        Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.


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